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oai:gredos.usal.es:10366/1620072025-04-30T20:39:47Zcom_10366_137028com_10366_4512com_10366_3823col_10366_137029

Abecia, José Alfonso Canto, Francisco Keller, Matthieu Palacios Riocerezo, Carlos Chemineau, Philippe Delgadillo, José Alberto 2025-01-20T11:10:27Z 2025-01-20T11:10:27Z 2022 Abecia, J. A., Canto, F., Keller, M., Palacios, C., Chemineau, P., y Delgadillo, J. A. (2022). Exposure of rams in sexual rest to sexually activated males in spring increases plasma LH and testosterone concentrations. Theriogenology, 192, 116-121. https://doi.org/10.1016/j.theriogenology.2022.08.035 0093-691X http://hdl.handle.net/10366/162007 10.1016/j.theriogenology.2022.08.035 1879-3231 Eight stimulating rams, and twelve stimulated rams, were used to determine whether a similar endocrine response to the introduction of sexually active males in spring in a flock of ewes is observed in a flock of rams. The stimulating rams (n = 4) were induced into a sexually active state by exposure to 2 months of long days (16 h light/d) (15 December-15 February). At the end of the long-day period, rams were returned to the natural photoperiod. Control-stimulating rams (n = 4) were kept under the natural photoperiod. On April 20, stimulated rams were divided into 2 groups, and joined with activated (ACT; n = 6) or control stimulating rams (C; n = 6). On the day of ram introduction, stimulated rams were blood sampled for 8 h at 20-min intervals, from 4 h before to 4 h after ram introduction, and next day from 24 to 28 h after ram introduction, and analyzed for plasma LH concentrations, and 10, 20 and 30 days after ram introduction to measure plasma testosterone levels. Mean (±SEM) plasma LH concentrations (ng/ml) of stimulated rams were similar during the 4 h before stimulating-ram introduction (ACT: 0.59 ± 0.03; C: 0.53 ± 0.04; P > 0.05). The introduction of the photoperiod-treated stimulating rams increased LH concentrations of stimulated rams during the 4 h after their introduction (1.14 ± 0.37) compared with the C group (0.51 ± 0.03; P < 0.05), especially during the first hour (ACT: 0.93 ± 0.16; C: 0.49 ± 0.03; P < 0.05), and during the blood sampling period 24–28 h after ram introduction (0.75 ± 0.07 vs. 0.58 ± 0.04; P < 0.05). Before the introduction of stimulating rams, the LH pulse frequencies and amplitudes did not differ between groups; however, LH pulsatility was higher at 4 h (0.58 ± 0.11 pulses/h; P < 0.05), and had trend to be higher 24 h (0.50 ± 0.06) (P = 0.10) after the introduction of the photoperiod-treated stimulating rams compared with the control-stimulating rams (0.29 ± 0.08 and 0.29 ± 0.10, respectively). As for LH pulses, there was an effect of group (P < 0.05) on LH amplitude, which presented a trend to be higher in ACT rams 4 h after ram introduction (1.68 ± 0.30; P < 0.10) and higher 24 h (1.07 ± 0.08; P < 0.05) after ram introduction, compared with LH amplitudes of C rams (0.71 ± 0.06 and 0.82 ± 0.07, respectively). Plasma testosterone concentrations of rams exposed to photoperiod-treated activated rams were higher than those of rams exposed to control-stimulating rams, at 4 h, 20 and 30 days after ram introduction (P < 0.05). In conclusion, sexually active rams in spring are able to stimulate LH and testosterone secretion of other rams in sexual rest, a phenomenon we called “ram-to-ram effect". eng Elsevier Male effect Gonadotropin Steroids Photoperiod Efecto masculino Gonadotropina Esteroides 2302.15 Hormonas Exposure of rams in sexual rest to sexually activated males in spring increases plasma LH and testosterone concentrations info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.1016/j.theriogenology.2022.08.035 info:eu-repo/semantics/openAccess Theriogenology 192 116 121