2026-09-14T15:32:34Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1696382026-02-25T12:15:32Zcom_10366_3992com_10366_3947com_10366_3946com_10366_3823col_10366_3993

Vaquero, Javier Judée, Florian Vallette, Marie Decauchy, Henri Arbelaiz, Ander Aoudjehane, Lynda Scatton, Olivier González Sánchez, María Ester Merabtene, Fatiha Augustin, Jérémy Housset, Chantal Dufour, Thierry Fouassier, Laura 2026-02-09T10:26:59Z 2026-02-09T10:26:59Z 2020 Vaquero, J., Judée, F., Vallette, M., Decauchy, H., Arbelaiz, A., Aoudjehane, L., Scatton, O., Gonzalez-Sanchez, E., Merabtene, F., Augustin, J., Housset, C, Dufour, T., Fouassier, L. (2020). Cold-Atmospheric Plasma Induces Tumor Cell Death in Preclinical In Vivo and In Vitro Models of Human Cholangiocarcinoma. Cancers: 12: 1280. http://hdl.handle.net/10366/169638 10.3390/cancers12051280 32438553 2072-6694 [EN]Through the last decade, cold atmospheric plasma (CAP) has emerged as an innovative therapeutic option for cancer treatment. Recently,we have set up a potentially safe atmospheric pressure plasma jet device that displays antitumoral properties in a preclinical model of cholangiocarcinoma (CCA), a rare and very aggressive cancer emerging from the biliary tree with few efficient treatments. In the present study, we aimed at deciphering the molecular mechanisms underlying the antitumor effects of CAP towards CCA in both an in vivo and in vitro context. In vivo, using subcutaneous xenografts into immunocompromised mice, CAP treatment of CCA induced DNA lesions and tumor cell apoptosis, as evaluated by 8-oxoguanine and cleaved caspase-3 immunohistochemistry, respectively. The analysis of the tumor microenvironment showed changes in markers related to macrophage polarization. In vitro, the incubation of CCA cells with CAP-treated culture media (i.e., plasma-activated media, PAM) led to a dose response decrease in cell survival. At molecular level, CAP treatment induced double-strand DNA breaks, followed by an increased phosphorylation and activation of the cell cycle master regulators CHK1 and p53, leading to cell cycle arrest and cell death by apoptosis. In conclusion, CAP is a novel therapeutic option to consider for CCA in the future. eng http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess Attribution 3.0 Unported Cholangiocarcinoma cold plasma innovative therapy tumor cells macrofagos plasma selectivity plama jet Cold-Atmospheric Plasma Induces Tumor Cell Death in Preclinical In Vivo and In Vitro Models of Human Cholangiocarcinoma info:eu-repo/semantics/article