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oai:gredos.usal.es:10366/1697062026-02-11T01:01:38Zcom_10366_3992com_10366_3947com_10366_3946com_10366_3823col_10366_3993

Rovira-Llopis, Susana Bañuls, Celia de Marañon, Aranzazu M. Díaz Morales, Noelia Jover, Ana Garzon, Sandra Rocha, Milagros Victor, Victor M. Hernandez-Mijares, Antonio 2026-02-10T12:28:18Z 2026-02-10T12:28:18Z 2017 Rovira-Llopis, S., Bañuls, C., de Marañon, A. M., Diaz-Morales, N., Jover, A., Garzon, S., Rocha, M., Victor, V. M., & Hernandez-Mijares, A. (2017). Low testosterone levels are related to oxidative stress, mitochondrial dysfunction and altered subclinical atherosclerotic markers in type 2 diabetic male patients. Free radical biology & medicine, 108, 155–162. https://doi.org/10.1016/j.freeradbiomed.2017.03.029 0891-5849 http://hdl.handle.net/10366/169706 10.1016/j.freeradbiomed.2017.03.029 28359952 [EN]Introduction: Low testosterone levels in men are associated with type 2 diabetes and cardiovascular risk. However, the role of testosterone in mitochondrial function and leukocyte-endothelium interactions is unknown. Our aim was to evaluate the relationship between testosterone levels, metabolic parameters, oxidative stress, mitochondrial function, inflammation and leukocyte-endothelium interactions in type 2 diabetic patients. Materials and methods: The study was performed in 280 male type 2 diabetic patients and 50 control subjects. Anthropometric and metabolic parameters, testosterone levels, reactive oxygen species (ROS) production, mitochondrial membrane potential, TNFα, adhesion molecules and leukocyte-endothelium cell interactions were evaluated. Results: Testosterone levels were lower in diabetic patients. Total and mitochondrial ROS were increased and mitochondrial membrane potential, SOD and GSR expression levels were reduced in diabetic patients. TNFα, ICAM-1 and VCAM-1 levels, leukocyte rolling flux and adhesion were all enhanced in diabetic patients, while rolling velocity was reduced. Testosterone levels correlated negatively with glucose, HOMA-IR, HbA1c, triglycerides, nonHDL-c, ApoB, hs-CRP and AIP, and positively with HDL-c and ApoA1. The multivariable regression model showed that HDL-c, HOMA-IR and age were independently associated with testosterone. Furthermore, testosterone levels correlated positively with membrane potential and rolling velocity and negatively with ROS production, VCAM-1, rolling flux and adhesion. Conclusions: Our data highlight that low testosterone levels in diabetic men are related to impaired metabolic profile and mitochondrial function and enhanced inflammation and leukocyte-endothelium cell interaction, which leaves said patients at risk of cardiovascular events. Fondo Europeo de Desarrollo Regional (FEDER), Instituto de Salud Carlos III (ISCIII), Ministerio de Economía y Competitividad, Generalitat Valenciana (Ministry of Education of the Valencian Regional Government), Fundación FISABIO application/pdf eng Elsevier Inc. Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Testosterona Diabetes tipo 2 Riesgo cardiovascular Mitocondria Leucocitos Estrés oxidativo Disfunción mitocondrial Testosterone Type 2 diabetes Cardiovascular risk Mitochondria Leukocytes Oxidative stress Mitochondrial dysfunction Diabetes Mellitus, Type 2 Cardiovascular Diseases Oxidative Stress Mitochondria Testosterone Risk Factors Leukocytes 3209 Farmacología 2410.10 Fisiología Humana 3205.02 Endocrinología 2403 Bioquímica 3207.04 Patología Cardiovascular diabetes mellitus tipo II enfermedades cardiovasculares mitocondrias estrés oxidativo factores de riesgo testosterona leucocitos Low testosterone levels are related to oxidative stress, mitochondrial dysfunction and altered subclinical atherosclerotic markers in type 2 diabetic male patients info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://doi.org/10.1016/j.freeradbiomed.2017.03.029 PI15/1424 PI16/1083 PI16/00301 CB06/04/0071 PROMETEO 2014/035 GV/2016/169 UGP15-193 Free Radical Biology and Medicine 108 155 162