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oai:gredos.usal.es:10366/1697322026-02-12T01:01:18Zcom_10366_3992com_10366_3947com_10366_3946com_10366_3823col_10366_3993

López-Domènech, Sandra Martínez-Herrera, Mayte Abad-Jiménez, Zaida Morillas, Carlos Escribano-López, Irene Díaz Morales, Noelia Bañuls, Celia Víctor, Víctor M. Rocha, Milagros Víctor, Víctor M 2026-02-11T11:46:42Z 2026-02-11T11:46:42Z 2019 López-Domènech, S., Martínez-Herrera, M., Abad-Jiménez, Z., Morillas, C., Escribano-López, I., Díaz-Morales, N., Bañuls, C., Víctor, V. M., & Rocha, M. (2019). Dietary weight loss intervention improves subclinical atherosclerosis and oxidative stress markers in leukocytes of obese humans. International journal of obesity (2005), 43(11), 2200–2209. https://doi.org/10.1038/s41366-018-0309-5 0307-0565 http://hdl.handle.net/10366/169732 10.1038/s41366-018-0309-5 30622308 1476-5497 [EN]The relationship between caloric restriction-mediated weight loss and the generation of ROS and its effects on atherosclerotic markers in obesity is not fully understood. Therefore, we set out to investigate whether dietary weight loss intervention improves markers of oxidative stress in leukocytes and subclinical parameters of atherosclerosis. This was an interventional study of 59 obese subjects (BMI > 35 kg/m2) who underwent 6 months of dietary therapy, including a 6-week very-low-calorie diet (VLCD) followed by an 18-week low-calorie diet (LCD). We determined clinical parameters, inflammatory markers-hsCRP, TNFα and NFκB -, oxidative stress parameters-total superoxide, glutathione, catalase activity and protein carbonyl groups-, soluble cellular adhesion molecules-sICAM, sP-selectin, sPSGL-1 -, myeloperoxidase (MPO), leukocyte-endothelium cell interactions-rolling flux, velocity and adhesion-and LDL subfractions, before and after the dietary intervention. After losing weight, an improvement was observed in the patients' anthropometric, blood pressure and metabolic parameters, and was associated with reduced inflammatory response (hsCRP, TNFα and NFκB). Oxidative stress parameters improved, since superoxide production and protein carbonyl content were reduced and antioxidant systems were enhanced. In addition, a significant reduction of subclinical markers of atherosclerosis-small and dense LDL particles, MPO, sP-selectin and leukocyte adhesion-and an increase in soluble PSGL-1 were reported. Our findings reveal that the improvement of subclinical atherosclerotic markers after dietary weight loss intervention is associated with a reduction of oxidative stress in leukocytes and inflammatory pathways, suggesting that these are the underlying mechanisms responsible for the reduced risk of cardiovascular disease in obese subjects after losing weight. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Attribution-NonCommercial-NoDerivatives 4.0 Internacional Cirugía bariátrica Obesidad mórbida Dinámica mitocondrial Estrés oxidativo Interacción leucocito-endotelio Pérdida de peso Bariatric surgery Morbid obesity Mitochondrial dynamics Oxidative stress Leukocyte–endothelium interaction Weight loss Dietary weight loss intervention improves subclinical atherosclerosis and oxidative stress markers in leukocytes of obese humans info:eu-repo/semantics/article