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oai:gredos.usal.es:10366/1699042026-02-20T01:02:14Zcom_10366_128023com_10366_3947com_10366_3946com_10366_3823col_10366_128025

2026-02-19T09:03:58Z urn:hdl:10366/169904 Effect of prenatal steroidal inhibition of sPLA2 in a rat model of preterm lung Remesal Escalero, Ana Belén Luca, Danielle de San Feliciano Martín, Laura Isidoro García, María Minucci, Angelo Pocino, Krizia Casas, Josefina Fabrias, Gemma Capoluongo, Ettore D. Ludeña de la Cruz, María Dolores Preterm lung Secretory phospholipase A2 Prenatal steroids [EN]Introduction: Secretory phospholipase A2 regulates surfactant catabolism and inflammatory cascade. This enzyme is correlated with compliance, oxygenation and major outcomes in various forms of acute respiratory failure. Steroids inhibit secretory phospholipase A2 in cell culture and are widely used to boost surfactant production before preterm delivery. No data are available about the effect of antenatal steroids on secretory phospholipase A2 in the offspring: we aimed to study this effect in a rat model of preterm lung. Material and methods: Fifteen pregnant Wistar rats were randomized to receive betamethasone, dexamethasone or placebo at 20 and 21 days gestation. Newborn rats were supported for 8 h and then sacrificed: lung tissue was analysed for secretory phospholipase A2 expression and activity, inflammatory mediators and protein content. Lipidomics was analysed using liquid chromatography-mass spectrometry. Results: Secretory phospholipase A2 expression was significantly reduced by antenatal steroids (p < 0.001). Secretory phospholipase A2 activity, TNFa and lysophosphatidylethanolamine, a product of phospholipase reaction, were lowest in betamethasone-treated rats (p < 0.001). There was a strong correlation between secretory phospholipase A2 activity and lysophosphatidylethanolamine (r ¼ 0.75; p ¼ 0.001) and this remained significant after adjustment for total proteins or phospholipids. Conclusions: Antenatal steroids decrease secretory phospholipase A2 in rat model of preterm lung. 2026-02-19T09:03:58Z 2026-02-19T09:03:58Z 2016 2099-12-31 info:eu-repo/semantics/article Remesal, A., De Luca, D., San Feliciano, L., Isidoro-Garcia, M., Minucci, A., Pocino, K., Casas, J., Fabrias, G., Capoluongo, E. D., & de la Cruz, D. L. (2016). Effect of prenatal steroidal inhibition of sPLA2 in a rat model of preterm lung. Pulmonary Pharmacology and Therapeutics, 36, 31-36. https://doi.org/10.1016/J.PUPT.2015.12.001 1094-5539 http://hdl.handle.net/10366/169904 10.1016/j.pupt.2015.12.001 spa https://doi.org/10.1016/J.PUPT.2015.12.001 FIS-PI 12/562 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier