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oai:gredos.usal.es:10366/1699062026-02-20T01:02:16Zcom_10366_128023com_10366_3947com_10366_3946com_10366_3823col_10366_128025

Blanco Álvarez, Elena Pérez Andrés, Martín Arriba Méndez, Sonia de Contreras Sanfeliciano, Teresa Criado García, Ignacio Pelak, Ondrej Serra Caetano, Ana Romero, Alfonso Puig, Noemí Remesal Escalero, Ana Belén Torrez Canizales, Juan López Granados, Eduardo Kalina, Tomas Sousa, Ana E. van Zelm, Menno van der Burg, Mirjam van Dongen, Jacques J. M. Orfao de Matos Correia e Vale, José Alberto 2026-02-19T09:29:16Z 2026-02-19T09:29:16Z 2018 2099-12-31 Blanco, E., Pérez-Andrés, M., Arriba-Méndez, S., Contreras-Sanfeliciano, T., Criado, I., Pelak, O., Serra-Caetano, A., Romero, A., Puig, N., Remesal, A., Torres Canizales, J., López-Granados, E., Kalina, T., Sousa, A. E., van Zelm, M., van der Burg, M., van Dongen, J. J. M., & Orfao, A. (2018). Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood. Journal of Allergy and Clinical Immunology, 141(6), 2208-2219. https://doi.org/10.1016/J.JACI.2018.02.017 0091-6749 http://hdl.handle.net/10366/169906 10.1016/j.jaci.2018.02.017 [EN]Background: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory Bcell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n 5 19) and peripheral blood (n 5 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG1, IgG2, IgG3, IgA1, and IgA2 were already detected in cord blood and newborns at very low counts, whereas CD271IgM11IgD1 MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG1, IgG3, and IgA1) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG2, IgG4, and IgA2) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG1, IgG2, IgG3, IgA1, and IgA2; until 2 to 4 years for IgD; and until 5 to 9 years for IgG4 and decreasing thereafter. For most IgH isotypes (except IgD and IgG4), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life. eng Elsevier Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Age distribution B cell Age distribution plasma cell Plasma Cells Immunity Flow Cytometry 2412 Inmunología células plasmáticas citometría de flujo inmunidad Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.1016/J.JACI.2018.02.017 EDU/346/2013 FIS PI12/00905- FEDER Journal of Allergy and Clinical Immunology 141 6 2208 2219.e16