2026-09-14T13:18:43Zhttps://gredos.usal.es/oai/requestoai:gredos.usal.es:10366/192212025-06-05T12:40:28Zcom_10366_139852com_10366_4512com_10366_3823col_10366_139853
Gestión del Repositorio Documental de la Universidad de Salamanca
author
Malvar Ferreras, Teresa
author
Martínez Ortega, María Montserrat
author
Padilla-García, Nélida
author
Lambourdière, Josie
author
Machon, Nathalie
2009-10-07T11:05:57Z
2009-10-07T11:05:57Z
2018-05
Padilla-García, N., Malvar-Ferreras, T., Lambourdière, J., Martínez-Ortega, M. M., & Machon, N. (2018). Microsatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next-generation sequencing and high-resolution melting analyses. Applications in plant sciences, 6(5), e01154. doi:10.1002/aps3.1154
http://hdl.handle.net/10366/19221
10.1002/aps3.1154
[EN]Premise of the Study
The tetraploid Veronica aragonensis (Plantaginaceae) is a narrow endemic to the Iberian Peninsula. Specific microsatellite markers were developed to investigate genetic structure and diversity.
Methods and Results
A total of 15 polymorphic markers were characterized on three populations of V. aragonensis, using a microsatellite‐enriched library on an Ion Torrent sequencer and high‐resolution melting (HRM) analyses to rapidly discard nonreliable, multicopy, and/or monomorphic loci. Allele number per locus ranged from one to five, and levels of observed heterozygosity per population varied from 0.142 ± 0.301 to 0.281 ± 0.369. Most primers also amplified in the closely related species V. rosea and in three subspecies of V. tenuifolia.
Conclusions
The species‐specific microsatellite markers developed here represent an essential tool to provide genetic information on the population level for V. aragonensis. The low levels of variation detected highlight the importance of continued efforts to improve conservation of the species
spa
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
High‐resolution melting (HRM) analyses
Microsatellites
Plantaginaceae
Polyploidy
Veronica aragonensis
Microsatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next‐generation sequencing and high‐resolution melting analyses
info:eu-repo/semantics/article
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URL
https://gredos.usal.es/bitstream/10366/19221/6/BIOCONS_Microsatellite.pdf
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https://gredos.usal.es/bitstream/10366/19221/5/DFA_Microscopicmodeling.pdf.pdf.txt
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https://gredos.usal.es/bitstream/10366/19221/9/BIOCONS_Microsatellite.pdf.txt
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