2024-03-28T11:13:02Zhttps://gredos.usal.es/oai/requestoai:gredos.usal.es:10366/1407512022-02-07T15:45:36Zcom_10366_133043com_10366_4576com_10366_3823col_10366_135272
A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK
Jaraíz-Rodríguez, Myriam
Tabernero, María Dolores
González-Tablas Pimenta, María
Otero Rodríguez, Álvaro
Orfao de Matos Correia e Vale, José Alberto
Medina Jiménez, José María
Tabernero Urbieta, María Aránzazu
Instituto de Investigación Biomédica de Salamanca
Connexin43 (CX43)
CNS
Malignant tumor
Human glioma
Stem Cell Migration
[EN] Connexin43 (CX43), a protein that forms gap junction channels and hemichannels in astrocytes, is downregulated in high-grade gliomas.
Its relevance for glioma therapy has been thoroughly explored; however, its positive effects on proliferation are counterbalanced by its
effects onmigration and invasion. Here,weshowthat a cell-penetrating peptide based onCX43(TAT-Cx43266-283) inhibited c-Src and focal
adhesion kinase (FAK) and upregulated phosphatase and tensinhomolog inglioma stem cells (GSCs) derived from patients. Consequently,
TAT-Cx43266-283 reduced GSC motility, as analyzed by time-lapse microscopy, and strongly reduced their invasive ability. Interestingly, we
investigated the effects of TAT-Cx43266-283 on freshly removed surgical specimens as undissociated glioblastoma blocks, which revealed a
dramatic reduction in the growth, migration, and survival of these cells. In conclusion, a region of CX43 (amino acids 266–283) exerts an
important anti-tumor effect in patient-derived glioblastoma models that includes impairment of GSC migration and invasion.
2020-01-31T11:25:41Z
2020-01-31T11:25:41Z
2020-01-31T11:25:41Z
2017
info:eu-repo/semantics/article
Jaraíz-Rodríguez, M., Tabernero, M. D., González-Tablas, M., Otero, A., Orfao, A., Medina, J. M., & Tabernero, A. (2017). A short region of connexin43 reduces human glioma stem cell migration, invasion, and survival through Src, PTEN, and FAK. Stem cell reports, 9(2), 451-463.
2213-6711
http://hdl.handle.net/10366/140751
10.1016/j.stemcr.2017.06.007
eng
http://dx.doi.org/10.1016/j.stemcr.2017.06.007
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional