TY  - JOUR 
AU  - García-Tuñón, Ignacio
AU  - Alonso-Pérez, Verónica
AU  - Vuelta Ramos, Elena
AU  - Pérez- Ramos, Sandra
AU  - Herrero García, María
AU  - Méndez, Lucía
AU  - Hernández Sánchez, Jesús María
AU  - Martín Izquierdo, Marta
AU  - Saldaña, Raquel
AU  - Sevilla, Julián
AU  - Sánchez Guijo Martín, Fermín
AU  - Hernández Rivas, Jesús María
AU  - Sánchez Martín, Manuel Adolfo
PY  - 2019
UR  - http://hdl.handle.net/10366/146291
AB  - [EN]CRISPR/Cas9 allows the generation of knockout cell lines and null zygotes by inducing site-specific double-stranded breaks. In most cases the DSB is repaired by non-homologous end joining, resulting in small nucleotide insertions or deletions that...
LA  - eng
PB  - Stefan Maas, National Institutes of Health, UNITED STATES
KW  - CRISPR/Cas9
KW  - Donor
KW  - sgRNAs
KW  - Cell lines
KW  - Genome
KW  - Nucleotide
KW  - Gene therapy
KW  - CRISPR-Cas Systems
KW  - Cell Line
KW  - Genome
TI  - Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
DO  - 10.1371/journal.pone.0216674
T2  - PLOS ONE
VL  - 14
M2  - e0216674
ER  -