TY - JOUR AU - Nieto, Blanca AU - Gaspar, Sonia G AU - Sapio, Russell T AU - Clavaín, Laura AU - Bustelo, Xosé R. AU - Pestov, Dimitri G AU - Dosil Castro, Mercedes PY - 2021 UR - http://hdl.handle.net/10366/168975 AB - [EN]Biochemical studies of the human ribosome synthesis pathway have been hindered by technical difficulties in obtaining intact preribosomal complexes from internal regions of the nucleolus. Here we provide a detailed description of an extraction... LA - eng PB - Taylor and Francis KW - Preribosome KW - Human nucleolus KW - pre-rRNA KW - ribosome maturation KW - ribosome assembly KW - preribosome purification KW - ribosome production KW - RNA KW - Ribosomal Proteins KW - Ribosomes KW - Humans KW - Cell Nucleolus KW - HCT116 Cells KW - HeLa Cells KW - RNA Precursors TI - Efficient fractionation and analysis of ribosome assembly intermediates in human cells DO - 10.1080/15476286.2021.1965754 T2 - RNA biology VL - 18 M2 - 182 ER -