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Titolo
Sox10 Expression in Goldfish Retina and Optic Nerve Head in Controls and after the Application of Two Different Lesion Paradigms
Autor(es)
Soggetto
Transcription factors
Neuroscience
Gene expression
Nervous system regeneration
Optic nerve diseases
Myelination
Oligodendroglia
Fecha de publicación
2016
Editore
Public Library of Science (New York)
Citación
Parrilla, M., León-Lobera, F., Lillo, C., Aévalo, R., Aijón, J., Lara, J.M., Velasco, A. (2016). Sox10 Expression in Goldfish Retina and Optic Nerve Head in Controls and after the Application of Two Different Lesion Paradigms. PlosONE, 11 (5), pp. 1-20
Resumen
[EN]The mammalian central nervous system (CNS) is unable to regenerate. In contrast, the
CNS of fish, including the visual system, is able to regenerate after damage. Moreover, the
fish visual system grows continuously throughout the life of the animal, and it is therefore an
excellent model to analyze processes of myelination and re-myelination after an injury.
Here we analyze Sox10+ oligodendrocytes in the goldfish retina and optic nerve in controls
and after two kinds of injuries: cryolesion of the peripheral growing zone and crushing of the
optic nerve. We also analyze changes in a major component of myelin, myelin basic protein
(MBP), as a marker for myelinated axons. Our results show that Sox10+ oligodendrocytes
are located in the retinal nerve fiber layer and along the whole length of the optic nerve.
MBP was found to occupy a similar location, although its loose appearance in the retina differed
from the highly organized MBP+ axon bundles in the optic nerve. After optic nerve
crushing, the number of Sox10+ cells decreased in the crushed area and in the optic nerve
head. Consistent with this, myelination was highly reduced in both areas. In contrast, after
cryolesion we did not find changes in the Sox10+ population, although we did detect some
MBP- degenerating areas. We show that these modifications in Sox10+ oligodendrocytes
are consistent with their role in oligodendrocyte identity, maintenance and survival, and we
propose the optic nerve head as an excellent area for research aimed at better understanding
of de- and remyelination processes.
URI
ISSN
1932-6203
DOI
10.1371/journal.pone.0154703
Versión del editor
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