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Titolo
Mesenchymal stromal cells (MSC) from JAK2+
myeloproliferative neoplasms differ from
normal MSC and contribute to the
maintenance of neoplastic hematopoiesis
Autor(es)
Soggetto
Mesenchymal stromal cells
Cell biology
Neoplastic hematopoiesis
Células madre
Neoplasias hematopoyeticas
Fecha de publicación
2017
Editore
Public Library of Science (New York)
Citación
Ramos TL, Sánchez-Abarca LI, Rosón- Burgo B, Redondo A, Rico A, Preciado S, et al. (2017) Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis. PLoS ONE 12(8): e0182470.
Resumen
[EN]There is evidence of continuous bidirectional cross-talk between malignant cells and bone
marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and
progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have
compared the function and gene expression profile of BM-MSC from healthy donors (HDMSC)
and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation
and differentiation capacity between both groups. However, BM-MSC from MPN
expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas
CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed
a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared
to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and
survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in
the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC.
Furthermore, MPN-MSC showed alteration in the expression of genes associated to the
maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation
of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients
differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic
cells
URI
ISSN
1932-6203
DOI
https://doi.org/10.1371/journal.pone.0182470
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