dc.contributor.author | Ramos, Teresa L. | |
dc.contributor.author | Sánchez Abarca, Luis Ignacio | |
dc.contributor.author | Rosón Burgo, Beatriz | |
dc.contributor.author | Redondo Guijo, Alba María | |
dc.contributor.author | Rico, Ana | |
dc.contributor.author | Preciado Pérez, Silvia | |
dc.contributor.author | Ortega, Rebeca | |
dc.contributor.author | Rodríguez, Concepción | |
dc.contributor.author | Muntión Olave, María Sandra | |
dc.contributor.author | Hernández Hernández, Ángel | |
dc.contributor.author | Rivas Sanz, Javier de las | |
dc.contributor.author | González, Marcos | |
dc.contributor.author | González Porras, José Ramón | |
dc.contributor.author | Cañizo Fernández-Roldán, María Consuelo del | |
dc.contributor.author | Sánchez-Guijo Martín, Fermín | |
dc.date.accessioned | 2017-09-21T08:23:36Z | |
dc.date.available | 2017-09-21T08:23:36Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Ramos TL, Sánchez-Abarca LI, Rosón- Burgo B, Redondo A, Rico A, Preciado S, et al. (2017) Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis. PLoS ONE 12(8): e0182470. | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10366/135282 | |
dc.description.abstract | [EN]There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HDMSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cells | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (New York) | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivs 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Mesenchymal stromal cells | es_ES |
dc.subject | Cell biology | es_ES |
dc.subject | Neoplastic hematopoiesis | es_ES |
dc.subject | Células madre | es_ES |
dc.subject | Neoplasias hematopoyeticas | es_ES |
dc.title | Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publishversion | https://doi.org/10.1371/journal. pone.0182470 | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0182470 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
Navegar
Todo o repositórioComunidades e ColeçõesPor data do documentoAutoresAssuntosTítulosEsta coleçãoPor data do documentoAutoresAssuntosTítulos