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Título
Variation and Distribution of L-A Helper Totiviruses in Saccharomyces sensu stricto Yeasts Producing Different Killer Toxins
Autor(es)
Materia
Cell and molecular biology
Yeast killer toxins
Microbiology
L-A totivirus
Double-stranded RNA virus
Saccharomyces sensu stricto
Fecha de publicación
2017
Editor
MDPI Publishing (Basilea, Suiza)
Citación
Rodríguez-Cousiño, N., Gómez, P., Esteban, R. (2017). Variation and Distribution of L-A Helper Totiviruses in Saccharomyces sensu stricto Yeasts Producing Different Killer Toxins. Toxins, 9 (10)
Resumen
[EN]Yeasts within the Saccharomyces sensu stricto cluster can produce different killer toxins.
Each toxin is encoded by a medium size (1.5–2.4 Kb) M dsRNA virus, maintained by a larger helper
virus generally called L-A (4.6 Kb). Different types of L-A are found associated to specific Ms: L-A in
K1 strains and L-A-2 in K2 strains. Here, we extend the analysis of L-A helper viruses to yeasts other
than S. cerevisiae, namely S. paradoxus, S. uvarum and S. kudriavzevii. Our sequencing data from nine
new L-A variants confirm the specific association of each toxin-producing M and its helper virus,
suggesting co-evolution. Their nucleotide sequences vary from 10% to 30% and the variation seems
to depend on the geographical location of the hosts, suggesting cross-species transmission between
species in the same habitat. Finally, we transferred by genetic methods different killer viruses from
S. paradoxus into S. cerevisiae or viruses from S. cerevisiae into S. uvarum or S. kudriavzevii. In the foster
hosts, we observed no impairment for their stable transmission and maintenance, indicating that the
requirements for virus amplification in these species are essentially the same. We also characterized
new killer toxins from S. paradoxus and constructed “superkiller” strains expressing them.
URI
ISSN
2072-6651
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