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Titolo
Effect of Angiotensin II and Small GTPase Ras Signaling Pathway Inhibition on Early Renal Changes in a Murine Model of Obstructive Nephropathy
Autor(es)
Soggetto
Obstructive Nephropathy
Small GTPase
Tubulointerstitial fibrosis
Unilateral ureteral obstruction (UUO)
Nefropatía obstructiva
Fibrosis tubulointersticial
Obstrucción ureteral unilateral (UUO)
Clasificación UNESCO
3209 Farmacología
3205.06 Nefrología
Fecha de publicación
2014
Editore
Hindawi Publishing Corporation (London, United Kingdom)
Resumen
[EN] Tubulointerstitial fibrosis is a major feature of chronic kidney disease. Unilateral ureteral obstruction (UUO) in rodents leads to the development of renal tubulointerstitial fibrosis consistent with histopathological changes observed in advanced chronic kidney disease in humans. The purpose of this study was to assess the effect of inhibiting angiotensin II receptors or Ras activation on early renal fibrotic changes induced by UUO. Animals either received angiotensin II or underwent UUO. UUO animals received either losartan, atorvastatin, and farnesyl transferase inhibitor (FTI) L-744,832, or chaetomellic acid A (ChA). Levels of activated Ras, phospho-ERK1/2, phospho-Akt, fibronectin, and 𝛼-smooth muscle actin were subsequently quantified in renal tissue by ELISA, Western blot, and/or immunohistochemistry. Our results demonstrate that administration of angiotensin II induces activation of the small GTPase Ras/Erk/Akt signaling system, suggesting an involvement of angiotensin II in the early obstruction-induced activation of renal Ras. Furthermore, upstream inhibition of Ras signalling by blocking either angiotensin AT1 type receptor or by inhibiting Ras prenylation (atorvastatin, FTI o ChA) reduced the activation of the Ras/Erk/Akt signaling systemand decreased the early fibrotic response in the obstructed kidney.This study points out that pharmacological inhibition of Ras activation may hold promise as a future strategy in the prevention of renal fibrosis.
URI
ISSN
2314-6133
DOI
10.1155/2014/124902
Versión del editor
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