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Título
Defining the Species Micromonospora saelicesensis and Micromonospora noduli Under the Framework of Genomics
Autor(es)
Palabras clave
Genome sequencing
Micromonospora
Nitrogen-fixing nodule
Phylogenomic analysis
Species delimitation
Taxonomy
Clasificación UNESCO
2409 Genética
2407 Biología Celular
Fecha de publicación
2018
Editor
Frontiers Media S.A.
Citación
Riesco, R., Carro, L., Román-Ponce, B., Prieto, C., Blom, J., Klenk, H. P., Normand, P., & Trujillo, M. E. (2018). Defining the species Micromonospora saelicesensis and Micromonospora noduli under the framework of genomics. Frontiers Media S.A., 9. https://doi.org/10.3389/fmicb.2018.01360
Resumen
[EN]The type isolates of species Micromonospora saelicesensis and Micromonospora noduli are Gram-stain positive actinobacteria that were originally isolated from nitrogen fixing nodules of the legumes Lupinus angustifolius and Pisum sativum, respectively. These two species are very closely related and questions arise as to whether they should be merged into a single species. To better delineate the relationship of M. saelicesensis and M. noduli, 10 strains isolated from plant tissue (nodules and leaves) and identified by their 16S rRNA gene sequences as either M. saelicensesis or M. noduli, based on a cut-offvalue of ≥99.5% were selected for whole-genome sequencing and compared with the type strains of M. saelicesensis Lupac 09T and M. noduli GUI43T using overall genome relatedness indices (OGRI) which included ANI, OrthoANI and digital DNA-DNA hybridization. Whole- and core-genome phylogenomic analyses were also carried out. These results were compared with the topologies of the 16S rRNA and gyrB gene phylogenies. Good correlation was found between all trees except for the 16S rRNA gene. Overall results also supported the current classification of M. saelicesensis and M. noduli as separate species.
URI
DOI
10.3389/fmicb.2018.01360
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