Compartir
Título
Sugar transport for enhanced xylose utilization in Ashbya gossypii
Autor(es)
Assunto
Sugar transport
Glucose
Xylose
Ashbya gossypii
CRISPR/Cas9
Clasificación UNESCO
3302.03 Microbiología Industrial
Fecha de publicación
2020
Resumen
[EN]The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant differences in the expression profile of the HXT homologs were found in response to different concentrations of sugars. More importantly, an amino acid replacement (N355V) in AFL205Cp, introduced by CRISPR/Cas9-mediated genomic edition, notably enhanced the utilization of xylose in the presence of glucose. Hence, the introduction of the afl205c-N355V allele in engineered strains of A. gossypii will further benefit the utilization of mixed sugars in this fungus.
URI
DOI
10.1007/s10295-020-02320-5
Versión del editor
Aparece en las colecciones
- GIIM. Artículos [7]