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Título
Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
Autor(es)
Assunto
Ashbya gossypii
Microbial lipids
Regulation
Lignocellulosic hydrolysates
Xylose
Molasses
Glycerol
Ashbya gossypii
Microbial lipids
Regulation
Lignocellulosic hydrolysates
Xylose
Molasses
Glycerol
Clasificación UNESCO
2414.06 Hongos
2409.02 Ingeniería Genética
Fecha de publicación
2019-08
Resumen
[EN]This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Δ9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.
URI
DOI
10.1016/j.biortech.2019.122054
Versión del editor
Aparece en las colecciones
- GIIM. Artículos [7]