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Título
Nucleoredoxin downregulation reduces β-Catenin levels and shifts hematopoietic differentiation towards myeloid lineage in vitro
Autor(es)
Palabras clave
Nucleoredoxin
β-catenin
Hematopoietic differentiation
Fecha de publicación
2021-04-04
Editor
MDPI
Citación
Pérez-Fernández, A., López-Ruano, G., Prieto-Bermejo, R., Sánchez-Bernal, C., Sánchez-Yagüe, J., & Hernández-Hernández, Á. (2021). Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro. BioChem, 1(1), 26-35. https://doi.org/10.3390/biochem1010003
Resumen
[EN]The importance of dissecting signaling pathways governing cell differentiation is based
on their relevance not only for understanding basic biological phenomena but also for better comprehending
the underlying mechanisms of pathologic alterations such as cancer. A paradigm of
cell differentiation processes is hematopoiesis, where a single stem cell gives rise to multiple, fully
differentiated, cell lineages. Nucleoredoxin (Nrx), a member of the thioredoxin family, is an important
redox-sensitive modulator of Wnt/ -catenin signaling, a key pathway for the control of
hematopoiesis. In this work, the relevance of Nrx for the differentiation of mouse hematopoietic
progenitor cells has been analyzed in vitro. Nrx silencing leads to a dramatic reduction in the size
of the Lin and LSK progenitor populations. Moreover, there is also a remarkable decrease in
CD3+ cells and an enhancement in the percentage of CD11b+Gr1 myeloid cells. This myeloid
bias would agree with the inhibition of the Wnt/ -catenin pathway. Interestingly, a reduction in
-catenin at the protein level was observed upon Nrx silencing. Our results strongly support the
importance of Nrx for hematopoietic differentiation, which could be mediated by the regulation of
the Wnt/ -catenin pathway.
URI
DOI
10.3390/biochem1010003
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