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Titel
Cultures of glial cells from optic nerve of two adult teleost fish: Astatotilapia burtoni and Danio rerio
Autor(es)
Schlagwort
Cell culture
Glial cells
Zebrafish
Cichlid
Optic nerve
Clasificación UNESCO
2490 Neurociencias
Fecha de publicación
2021
Verlag
Elsevier
Citación
DeOliveira-Mello, L., Mack, A. F., Lara, J. M., & Arévalo, R. (2021). Cultures of glial cells from optic nerve of two adult teleost fish: Astatotilapia burtoni and Danio rerio. Journal of Neuroscience Methods, 353, 109096. https://doi.org/10.1016/j.jneumeth.2021.109096
Resumen
[EN]Background: In vitro studies are very useful to increase the knowledge of different cell types and could be the key
to understand cell metabolism and function. Fish optic nerves (ON) can recover visual functions by reestablishing
its structure and reconnecting the axons of ganglion cells. This is because fish show spontaneous regeneration of
the central nervous system which does not occur in mammals. In addition, several studies have indicated that
glial cells of ON have different properties in comparison to the glial cells from brain or retina. Consequently,
providing an in vitro tool will be highly beneficial to increase the knowledge of these cells.
New Method: We developed a cell culture protocol to isolate glial cells from ON of two teleost fish species, Danio
rerio and Astatotilapia burtoni.
Results: The optimized protocol allowed us to obtain ON cells and brain-derived cells from adult teleost fish.
These cells were characterized as glial cells and their proprieties in vitro were analyzed.Comparison with Existing
Method(s): Although it is striking that ON glial cells show peculiarities, their study in vitro has been limited by the
only published protocol going back to the 1990s. Our protocol makes glial cells of different fish species available
for experiments and studies to increase the understanding of these glial cell types.
Conclusions: This validated and effective in vitro tool increases the possibilities on studies of glial cells from fish
ON which implies a reduction in animal experimentation.
URI
ISSN
0165-0270
DOI
10.1016/J.JNEUMETH.2021.109096
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