dc.contributor.author | Orfao de Matos Correia e Vale, José Alberto | |
dc.contributor.author | García Sanz, Ramón | |
dc.contributor.author | López‐Berges, M. Consuelo | |
dc.contributor.author | Vidriales Vicente, María Belén | |
dc.contributor.author | González Díaz, Marcos | |
dc.contributor.author | Caballero Barrigón, María Dolores | |
dc.contributor.author | San Miguel Izquierdo, Jesús Fernando | |
dc.date.accessioned | 2024-02-03T16:03:09Z | |
dc.date.available | 2024-02-03T16:03:09Z | |
dc.date.issued | 1994-12-01 | |
dc.identifier.citation | Orfão, A., García‐Sanz, R., López‐Berges, M. C., Vidriales, M. B., González, M., Caballero, M. D., & San Miguel, J. F. (1994). A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique. Cytometry: The Journal of the International Society for Analytical Cytology, 17(4), 332-339. DOI: 10.1002/cyto.990170409 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10366/155250 | |
dc.description | Este trabajo generó la validación de una técnica para estudiar la fase de síntesis de las células plasmáticas en mieloma múltiple y gammapatías monoclonales de significado incierto. Su desarrollo permitió luego aplicar la técnica a una serie de pacientes con mieloma múltiple y determinar su valor pronóstico. Fue la base de la tesis del Dr. García-Sanz permitiendo iniciar una carrera y una línea de investigación del Servicio de hematología que actualmente ha permitido a dicho servicio ser la referencia nacional en esta enfermedad, incluyendo tanto el desarrollo del programa de trasplante, los ensayos clínicos y la línea de citometría de flujo del servicio. | es_ES |
dc.description.abstract | [EN]In the present paper a CD38/propidium iodide double staining technique is described which separately assesses the cell cycle distribution of myelomatous plasma cells from that of the residual normal hemopoietic cells. For this purpose, bone marrow (BM) cells from a group of 42 untreated multiple myeloma patients were analyzed. Of these, 23 cases were aneuploid (55%) and 19 diploid (45%). The use of the CD38/propidium iodide double staining method allowed a clear separation between CD38 strong positive cells from the remaining bone marrow populations, cell sorting experiments confirming that plasma cells were almost exclusively contained in the former fraction where they represented 97 +/- 2% of the total cells sorted. In all cases, the S-phase in plasma cells and in the remaining normal hemopoietic bone marrow cells was assessed, being higher in normal hemopoietic cells (8.0 +/- 6.3%) than in plasma cells (3.3 +/- 2.6%, P < 0.002). In addition, there was no correlation between the S-phase of the neoplastic and normal bone marrow cells (r = 0.22; P > 0.10); this work therefore shows that the assessment of the total proliferative rate of bone marrow samples does not reflect either the proliferation of normal cells or that of neoplastic plasma cells but will depend on the proliferative rate and the percentage of each population within the sample, which can be assessed by the technique described here. | es_ES |
dc.description.sponsorship | Hospital Universitario de Salamanca Universidad de Salamanca | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Flok Cytometry | es_ES |
dc.subject | Plasma cells | es_ES |
dc.subject | CD38 | es_ES |
dc.subject | Proliferative activity | es_ES |
dc.subject.mesh | Immunophenotyping | * |
dc.subject.mesh | Aged | * |
dc.subject.mesh | Bone Marrow | * |
dc.subject.mesh | Staining and Labeling | * |
dc.subject.mesh | Cell Cycle | * |
dc.subject.mesh | DNA | * |
dc.subject.mesh | Flow Cytometry | * |
dc.subject.mesh | Humans | * |
dc.subject.mesh | Propidium | * |
dc.subject.mesh | Middle Aged | * |
dc.subject.mesh | Plasma Cells | * |
dc.subject.mesh | Multiple Myeloma | * |
dc.subject.mesh | Antibodies | * |
dc.title | A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publishversion | https://doi.org/10.1002/cyto.990170409 | es_ES |
dc.subject.unesco | 3205.04 Hematología | es_ES |
dc.identifier.doi | 10.1002/cyto.990170409 | |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.identifier.essn | 1552-4930 | |
dc.journal.title | Cytometry Part A | es_ES |
dc.volume.number | 17 | es_ES |
dc.issue.number | 4 | es_ES |
dc.page.initial | 332 | es_ES |
dc.page.final | 339 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.decs | humanos | * |
dc.subject.decs | células plasmáticas | * |
dc.subject.decs | tinción y etiquetado | * |
dc.subject.decs | anciano | * |
dc.subject.decs | mediana edad | * |
dc.subject.decs | mieloma múltiple | * |
dc.subject.decs | inmunofenotipificación | * |
dc.subject.decs | ciclo celular | * |
dc.subject.decs | ADN | * |
dc.subject.decs | citometría de flujo | * |
dc.subject.decs | propidio | * |
dc.subject.decs | anticuerpos | * |
dc.subject.decs | médula ósea | * |
dc.description.project | Hospital Universitario de Salamanca | es_ES |
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