Compartir
Titre
A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique
Autor(es)
Sujet
Flok Cytometry
Plasma cells
CD38
Proliferative activity
Clasificación UNESCO
3205.04 Hematología
Fecha de publicación
1994-12-01
Éditeur
Wiley
Citación
Orfão, A., García‐Sanz, R., López‐Berges, M. C., Vidriales, M. B., González, M., Caballero, M. D., & San Miguel, J. F. (1994). A new method for the analysis of plasma cell DNA content in multiple myeloma samples using a CD38/propidium iodide double staining technique. Cytometry: The Journal of the International Society for Analytical Cytology, 17(4), 332-339. DOI: 10.1002/cyto.990170409
Resumen
[EN]In the present paper a CD38/propidium iodide double staining technique is described which separately assesses the cell cycle distribution of myelomatous plasma cells from that of the residual normal hemopoietic cells. For this purpose, bone marrow (BM) cells from a group of 42 untreated multiple myeloma patients were analyzed. Of these, 23 cases were aneuploid (55%) and 19 diploid (45%). The use of the CD38/propidium iodide double staining method allowed a clear separation between CD38 strong positive cells from the remaining bone marrow populations, cell sorting experiments confirming that plasma cells were almost exclusively contained in the former fraction where they represented 97 +/- 2% of the total cells sorted. In all cases, the S-phase in plasma cells and in the remaining normal hemopoietic bone marrow cells was assessed, being higher in normal hemopoietic cells (8.0 +/- 6.3%) than in plasma cells (3.3 +/- 2.6%, P < 0.002). In addition, there was no correlation between the S-phase of the neoplastic and normal bone marrow cells (r = 0.22; P > 0.10); this work therefore shows that the assessment of the total proliferative rate of bone marrow samples does not reflect either the proliferation of normal cells or that of neoplastic plasma cells but will depend on the proliferative rate and the percentage of each population within the sample, which can be assessed by the technique described here.
Description
Este trabajo generó la validación de una técnica para estudiar la fase de síntesis de las células plasmáticas en mieloma múltiple y gammapatías monoclonales de significado incierto. Su desarrollo permitió luego aplicar la técnica a una serie de pacientes con mieloma múltiple y determinar su valor pronóstico. Fue la base de la tesis del Dr. García-Sanz permitiendo iniciar una carrera y una línea de investigación del Servicio de hematología que actualmente ha permitido a dicho servicio ser la referencia nacional en esta enfermedad, incluyendo tanto el desarrollo del programa de trasplante, los ensayos clínicos y la línea de citometría de flujo del servicio.
URI
DOI
10.1002/cyto.990170409
Versión del editor
Aparece en las colecciones
Patrocinador
Hospital Universitario de Salamanca
Fichier(s) constituant ce document
Nombre:
1994-7533074 Orfao, Cytometry.pdfEmbargado hasta: 2099-09-09
Tamaño:
173.9Ko
Formato:
Adobe PDF
Descripción:
Original Article