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Título
Immunolocalization of a Cell Wall ß-Galactosidase Reveals its Developmentally Regulated Expression in Cicer arietinum and its Relationship to Vascular Tissue
Autor(es)
Palabras clave
Cell wall
Chickpea
Collenchyma
Development
ß-Galactosidase
Vascular tissue
Fecha de publicación
2008
Resumen
We report the generation of antibodies against
a ß-galactosidase from Cicer arietinum, ßIV-Gal, and the
subsequent immunolocalization of the protein in different
parts and developmental stages of the plant. The ßIV-Gal
protein is encoded by the CanBGal-4 gene, which belongs
to a family of at least four ß-galactosidase genes, transcripts
of which were previously reported to be mainly
present in seedling epicotyls and plant stem, its transcription
pattern being inversely related to elongation rate of
these organs. ßIV-Gal protein was detected in the cell walls
of seedling epicotyls and plant stems. The immunodetection
of ßIV-Gal protein in the cell wall protein extracts
from aged epicotyls and basal stem internodes, both
undergoing low rates of elongation, is in agreement with
the trend of the CanBGal-4 transcript and indicates a
relationship of this cell wall protein with the end of cell
elongation. The specific main location of the ßIV-Gal
protein in vascular tissue of epicotyls and stems and in a
layer of sclerenchymatic cells surrounding the vascular
cylinder (perivascular fibers) allows us to postulate a
function for this ß-galactosidase in the modification of cell
wall polymers during the development of cells of the
vascular system. The localization of the ßIV-Gal protein
also in the cell walls of collenchyma cells in internodes is
consistent with the involvement of ßIV-Gal in cell wall modifications that lead to thick cell walls, such as in vascular
cells.
URI
ISSN
0721-7595
DOI
10.1007/s00344-008-9044-9
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- GIPACYCV. Artículos [59]
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