dc.contributor.author | Dopico, Berta | |
dc.contributor.author | Nicolás Rodrigo, Gregorio | |
dc.contributor.author | Labrador, Emilia | |
dc.date.accessioned | 2024-04-16T08:10:51Z | |
dc.date.available | 2024-04-16T08:10:51Z | |
dc.date.issued | 1989 | |
dc.identifier.issn | 0031-9317 | |
dc.identifier.uri | http://hdl.handle.net/10366/157393 | |
dc.description.abstract | Two protein fractions with activity as a-galactosidase (EC 3.2.1.22) and a-arabinosidase (EC 3.2.1.55), respectively, were identified in the proteins of cell wall of Cicer arietinum L. cv. Castellana extracted with 3 M LiCI. These fractions were partially purified by gel filtration chromatography (Bio Gel P-150), increasing the specific arabinosidase activity 57-fold and the a-galactosidase activity 6-fold. Other protein fractions with glucosidase (EC 3.2.1.21) and glucanase (EC 3.2.1.6) activity also appeared. According to earlier authors, a-arabinosidases and a-galactosidases are related to alterations in linkages occurring in cell walls, since the enzymes are able to hydrolyze isolated wall polymers. However, our preparations hydrolyze intact cell walls only to a very limited extent, such that their participation in the autolytic processes of cell walls can be ruled out. | es_ES |
dc.description.sponsorship | This work was supported by a grant from Comisión Asesora de Investigación Científica y Técnica, Spain no 0395-84. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | a-arabinosidase | es_ES |
dc.subject | Cell wall | es_ES |
dc.subject | Cicer arietinum | es_ES |
dc.subject | Epicotyls | es_ES |
dc.subject | a-galactosidase | es_ES |
dc.subject | Glucanase | es_ES |
dc.title | Partial purification of cell wall α‐galactosidases and α‐arabinosidases from Cicer arietinum epicotyls. Relationship with cell wall autolytic processes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1111/j.1399-3054.1989.tb05610.x | |
dc.relation.projectID | 0395-84 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.identifier.essn | 1399-3054 | |
dc.journal.title | Physiologia Plantarum | es_ES |
dc.volume.number | 75 | es_ES |
dc.issue.number | 4 | es_ES |
dc.page.initial | 465 | es_ES |
dc.page.final | 468 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |