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Titel
Variations of nNOS and its mRNA in the neurons of the hypothalamic supraoptic nucleus in response to stress by immobilization
Autor(es)
Schlagwort
Nitric oxide
Stress
In situ hybridization
Immunohistochemistry
Optical densitometry
Morphometry
Fecha de publicación
2009
Resumen
[EN]Using rats subjected to immobilization stress,
the aim of the present study was to investigate
the expression of neuronal nitric oxide synthase
and of its mRNA in the magnocellular
neurons of the supraoptic nucleus of the hypothalamus,
which does not have parvocellular
neurons or direct neurohaemal connections
with the anterior lobe of the hypophysis.
nNOS expression was studied with
immunohistochemistry using sheep antinNOS
serum, and the intraneural detection of
nNOS mRNA was accomplished using a nonisotopic
in situ hybridization technique,
employing a specific biotinylated probe. The
acute stress elicited by restraint induced an
increase in the overall size of the supraoptic
nucleus, together with an increase in the number
of magnocellular neurons expressing
nNOS immunoreaction. The optical densitometry
values of the nNOS immunoreaction,
the nuclear areas of the immunoreactive neurons,
and the density of neurons showing
nNOS mRNA hybrids were higher in the
supraoptic nucleus of the stress-treated animals
than in the controls, suggesting that
nitric oxide of nNOS origin plays an active
role in the magnocellular neurons of the
hypophyseal-hypothalamic system and the
involvement of this system in the stress axis.
URI
ISSN
1136-4890
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