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Título
Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
Autor(es)
Assunto
Opioid receptors
Antibodies
Zebrafish
Immunology
Fecha de publicación
2018
Citación
Arévalo, J. C., Hernández-Jiménez, E., Jiménez-González, A., Torres-Valle, M., Iwasaki, R. S., López-Bellido, R., ... & Rodríguez, R. E. (2018). Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish. International journal of molecular sciences, 19(1), 14.
Resumen
[EN] The opioid system is well conserved among species and plays a critical role in pain
and addiction systems. The use of zebrafish as an experimental model to study development and
genetics is extraordinary and has been proven to be relevant for the study of different diseases.
The main drawback to its use for the analysis of different pathologies is the lack of protein tools.
Antibodies that work in other models are not suitable for zebrafish due to the low degree of homology
that exists among the opioid receptor protein sequences in different species. Here we report the
successful generation and characterization of antibodies against the mu, delta 1 and delta 2 opioid
receptors in zebrafish. The antibodies obtained, which are specific for each receptor due to the use
of the C-terminus as antigens, work for Western blotting and immunohistochemistry. In addition,
the antibodies against mu and delta 1 opioid receptors, but not those against delta 2, are able to
immunoprecipitate the corresponding receptor from zebrafish lysates. The development of opioid
receptor antibodies is an asset to the further study of the endogenous opioid system in zebrafish.
URI
DOI
10.3390/ijms19010014
Versión del editor
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