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Título
Broad-range potential of Asphodelus microcarpus leaves extract for drug development
Autor(es)
Materia
Asphodelus microcarpus
Plant extract
Antibiotics
Biofilm
Antimicrobial activity
Antiviral response
Ebola virus
Pharmaceuticals
Fecha de publicación
2017
Citación
Di Petrillo, A., Fais, A., Pintus, F., Santos-Buelga, C., González-Paramás, A. M., Piras, V., ... & Frau, A. (2017). Broad-range potential of Asphodelus microcarpus leaves extract for drug development. BMC microbiology, 17(1), 159.
Resumen
[EN]
Background: Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal,
antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities.
In order to find novel antimicrobial and antiviral agents, the aim of the present study was the evaluation of the
antibacterial and antibiofilm susceptibility of Asphodelus microcarpus leaves extract. Moreover, the antiviral activity
and the phytochemical composition of the active extract were also determined.
Methods: Antimicrobial and antibiofilm activities of leaves ethanol extract of A. microcarpus were evaluated on 13
different microbial strains. We selected three different sets of microorganisms: (i) Gram-positive bacteria, (ii) Gramnegative
bacteria and (iii) yeasts. The potential antiviral activity of A. microcarpus leaves ethanol extract was evaluated
with a luciferase reporter gene assay in which the dsRNA-dependent RIG-I-mediated IFN-β activation was inducted or
inhibited by the Ebola virus VP35 protein. HPLC-DAD-MS was used to identify phenolic profile of the active extract.
Results: A. microcarpus leaves extract showed a potent inhibitory activity on Gram-positive bacteria while only a
reduced inhibition was observed on Gram-negative bacteria. No activity was detected against Yeasts. The extract
also showed an interesting antibiofilm motif on various bacterial strains (E. coli, S. aureus, S. haemolyticus and B. clausii).
Moreover, this extract significantly affected the Ebola virus VP35 inhibition of the viral RNA (vRNA) induced IFN response.
Conclusions: The overall results provide supportive data on the use of A. microcarpus as antimicrobial agent and a
potential source of anti-viral natural products.
Data collected set the bases for further studies for the identification of single active components and the development
of new pharmaceuticals.
URI
ISSN
1471-2180
DOI
10.1186/s12866-017-1068-5
Versión del editor
Colecciones
- DQANB. Artículos [61]