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Titolo
NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application
Autor(es)
Soggetto
Ammonium urea
Urease
Urea hydrolysis
NQS-PDMS sensor
Optical sensor
Glass support
In-situ analysis
Water
Urine
Clasificación UNESCO
2301 Química Analítica
Fecha de publicación
2021
Editore
MDPI
Resumen
[EN] The development of in situ analytical devices has gained outstanding scientific interest. A solid sensing membrane composed of 1,2-naphthoquinone-4-sulfonate (NQS) derivatizing reagent embedded into a polymeric polydimethylsiloxane (PDMS) composite was proposed for in situ ammonium (NH4+) and urea (NH2CONH2) analysis in water and urine samples, respectively. Satisfactory strategies were also applied for urease-catalyzed hydrolysis of urea, either in solution or glass-supported urease immobilization. Using diffuse reflectance measurements combined with digital image processing of color intensity (RGB coordinates), qualitative and quantitative analyte detection was assessed after the colorimetric reaction took place inside the sensing membrane. A suitable linear relationship was found between the sensor response and analyte concentration, and the results were validated by a thymol-PDMS-based sensor based on the Berthelot reaction. The suggested sensing device offers advantages such as rapidity, versatility, portability, and employment of non-toxic reagents that facilitate in situ analysis in an energy-efficient manner.
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- DQANB. Artículos [61]
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