Título
Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography
Autor(es)
Palabras clave
QuEChERS
LC
Tocopherols
Phytosterols
Nuts
Clasificación UNESCO
Fecha de publicación
2016
Editor
Elsevier
Citación
M. Milagros Delgado-Zamarreño, Cristina Fernández-Prieto, Myriam Bustamante-Rangel, Lara Pérez-Martín,
Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography,
Food Chemistry,
Volume 192,
2016,
Pages 825-830,
ISSN 0308-8146,
https://doi.org/10.1016/j.foodchem.2015.07.083.
(https://www.sciencedirect.com/science/article/pii/S0308814615011164)
Resumen
[EN]In the present work a simple, reliable and affordable sample treatment method for the simultaneous
analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried
out using the QuEChERS methodology and analyte separation and detection were accomplished using
HPLC–DAD. The use of this methodology for the extraction of natural occurring substances provides
advantages such as speed, simplicity and ease of use. The parameters evaluated for the validation of
the method developed included the linearity of the calibration plots, the detection and quantification limits, repeatability, reproducibility and recovery. The proposed method was successfully applied to the
analysis of tocopherols and free phytosterols in samples of almonds, cashew nuts, hazelnuts, peanuts,
tiger nuts, sun flower seeds and pistachios
URI
ISSN
0308-8146
DOI
10.1016/j.foodchem.2015.07.083
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