Título
Analysis of interaction between dendriplexes and bovine serum albumin.
Autor(es)
Palabras clave
Base Sequence
DNA Primers
Dendrimers
Serum Albumin, Bovine
Spectrometry, Fluorescence
Fecha de publicación
2007-07
Citación
Shcharbin, D., Pedziwiatr, E., Chonco, L., Bermejo-Martín, J. F., Ortega, P., de la Mata, F. J., ... & Muñoz-Fernandez, M. A. (2007). Analysis of interaction between dendriplexes and bovine serum albumin. Biomacromolecules, 8(7), 2059-2062.
Resumen
[EN] Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTGTCTCCGCTTCTTCCTGCCA; unlabeled or fluorescein-labeled), novel water-soluble carbosilane dendrimers, and bovine serum albumin were studied by fluorescence and gel electrophoresis. The molar ratios of the dendrimer/ODN dendriplexes ranged from 4 to 7. The efficiency of formation and stability of the dendriplexes depended on electrostatic interactions between the dendrimer and the ODNs. Dendriplex formation significantly decreased the interactions between ODNs and albumin. Thus, the formation of dendriplexes between carbosilane dendrimers and ODNs may improve ODN delivery.
URI
ISSN
1525-7797
DOI
Versión del editor
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