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Título
Separation of pyranoanthocyanins from red wine by column chromatography
Autor(es)
Materia
Anthocyanin-derived pigments
Wine colour
Size exclusion chromatography
Liquid chromatography-mass spectrometry
Química analítica
Analytic chemistry
Fecha de publicación
2004
Citación
Alcalde-Eon, C., Escribano-Bailón, M. T., Santos-Buelga, C., & Rivas-Gonzalo, J. C. (2004). Separation of pyranoanthocyanins from red wine by column chromatography. Analytica Chimica Acta, 513(1), 305-318.
Resumen
[EN] With the aim of monitoring the formation of anthocyanin-derived pigments and contributing to the study of their chromatic properties,
stability and relative contribution to the colour of red wines, a method for fractionation of the colouring material was set up. The method
was based on the distinct reactivity of the different pigment families towards bisulfite (hydrogen sulfite). The wine, acidified and bleached
with NaHSO3, was placed in a Toyopearl® HW-40(s) gel column and submitted to elution with ethanol. Two fractions with different pigment
compositions were collected and analysed by liquid chromatographay diode array detection-mass spectrometry. Compounds present in
each fraction were identified according to their UV-visible and MSn mass spectra, showing that the first one was mostly constituted of
pyranoanthocyanins, whereas the second basically contained anthocyanins and anthocyanin-flavanol condensation products. A large variety
of new pigments were detected, some of which had not been previously reported in red wines, as far as we know. Characteristic MS2 and
MS3 fragmentation patterns were observed within each family of compounds, which could be further applied for characterisation of unknown
pigments in other wines.
URI
ISSN
0003-2670
DOI
10.1016/j.aca.2003.10.076
Versión del editor
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- GIP. Artículos [86]
- DQANB. Artículos [61]